Following the construction of a series of pSV2-cat derived plasmids containing the chloramphenicol acetyltransferase (CAT) gene under the control of a eukaryotic trout protamine promoter, it was noted thatEscherichia coli, transformed with these plasmids, developed resistance to chloramphenicol (CM). This result suggested that the eukaryotic trout protamine promoter possessed significant prokaryotic promoter activity. Modification of the trout protamine promoter region by removing the region containing the eukaryotic Goldberg-Hogness box in the plasmid p525-cat increased the expression of the CAT gene almost to the wild-type level and conferred strong CM resistance. Sequence comparisons of the plasmid series indicate that prokaryotic promoter elements are present in the trout protamine promoter and that their similarity to the prokaryotic promoter consensus sequences and the distance between the two elements is more favourable in p525-cat, the plasmid which conlers the greatest CM resistance.
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Research Article|
June 01 1985
Functional prokaryotic gene control signals within a eukaryotic rainbow trout protamine promoter
Jacek M. Jankowski;
Jacek M. Jankowski
1Department of Medical Biochemistry, Faculty of Medicine, Health Sciences Centre, The University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1, Canada
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Eva Walczyk;
Eva Walczyk
1Department of Medical Biochemistry, Faculty of Medicine, Health Sciences Centre, The University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1, Canada
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Gordon H. Dixon
Gordon H. Dixon
1Department of Medical Biochemistry, Faculty of Medicine, Health Sciences Centre, The University of Calgary, 3330 Hospital Drive N.W., Calgary, Alberta T2N 4N1, Canada
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Publisher: Portland Press Ltd
Received:
May 13 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (6): 453–461.
Article history
Received:
May 13 1985
Citation
Jacek M. Jankowski, Eva Walczyk, Gordon H. Dixon; Functional prokaryotic gene control signals within a eukaryotic rainbow trout protamine promoter. Biosci Rep 1 June 1985; 5 (6): 453–461. doi: https://doi.org/10.1007/BF01116942
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