The13C isotopic labeling pattern in the disaccharide trehalose (1,1-α-α-D-glucose) produced by the micro-organismBrevibacterium flavum when grown on a medium containing [1-{au13}C]glucose has been determined. Long range coupling between C-1 and C-6 carbons of the glucose units can be observed in the excreted material. It is proposed that some of the13C isotopomers in the excreted trehalose reflect the labeling pattern in (unobserved) fructose 1,6-diphosphate. Analysis of the label distribution within the framework of a steady state kinetic model allows an analysis of the contributions of the hexose monophosphate shunt and the degree of equilibration of triose phosphate isomerase. Analogous measurements on excreted glucose could be carried out in other organisms.
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June 01 1985
Biosynthesis of trehalose by Brevibacterium flavum: Use of long range13C-13C coupling data to characterize triose phosphate isomerase activity
Robert E. London;
Robert E. London
1Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, P.O. Box 12233, Research Triangle Park, North Carolina 27709, USA
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Thomas E. Walker
Thomas E. Walker
2Los Alamos National Laboratory, Los Alamos, New Mexico, USA
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Publisher: Portland Press Ltd
Received:
June 11 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (6): 509–515.
Article history
Received:
June 11 1985
Citation
Robert E. London, Thomas E. Walker; Biosynthesis of trehalose by Brevibacterium flavum: Use of long range13C-13C coupling data to characterize triose phosphate isomerase activity. Biosci Rep 1 June 1985; 5 (6): 509–515. doi: https://doi.org/10.1007/BF01116950
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