An ontogenetic study of the effect of various neuhormones and other activators on adenylate cyclase systems was carried out using cultures of ceils from 15-d-old embryonic mouse brain. Dopamine stimulated the enzyme activity at earlier culture ages (i.e. 4 and 10 d) but had little stimulatory effect at later ages (i.e. 20 and 33 d). Further, this stimulation at the earlier ages was blocked by the dopaminergic blocker, fluphenazine, but not by α and β-adrenergic antagonists. In contrast to dopamine, isoproterenol (a β-adrenergic agonist) had little stimulatory effect at earlier ages, but its ability to stimulate cyclase activity increased with age. This increase in all age groups was blocked by propranolol (afg – adrenergic antagonists). Epinephrine-sensitive enzyme activity showed a steady increase with age, which could be blocked with propranolol except in β-d-old cultures, where it was blocked instead by fluphenazine. Because the cultures are relatively enriched in neurons at earlier ages and in glia in later ages, the results suggest a predominantly neuronal localization for the dopamine sensitive adenylate cyclases and a glial localization of the isoproterenol and epinephrine sensitive adenylate cyclases. Histamine, serotonin, calcium/calmodulin and chloroadenosine were either only slightly or not at all stimulatory.
Skip Nav Destination
Article navigation
Research Article|
July 01 1985
Developmental study on the regulation of neurotransmitter-sensitive adenylate cyclase systems in primary cerebral cell cultures from embryonic mice
Gouri Shanker;
Gouri Shanker
1Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140, USA
Search for other works by this author on:
Ronald A. Pieringer
Ronald A. Pieringer
1Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140, USA
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 28 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (7): 601–608.
Article history
Received:
June 28 1985
Citation
Gouri Shanker, Ronald A. Pieringer; Developmental study on the regulation of neurotransmitter-sensitive adenylate cyclase systems in primary cerebral cell cultures from embryonic mice. Biosci Rep 1 July 1985; 5 (7): 601–608. doi: https://doi.org/10.1007/BF01117073
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |