A two-base deletion mismatch was generated in a DNA heteroduplex by hybridization of two linear plasmid DNA molecules differing only by the presence of a two-base deletion in one of them. The heteroduplex was shown to be sensitive to double-strand cleavage by nuclease S1, thus demonstrating the potential value of single-stranded probes for the detection of polymorphisms in genomic DNA due to very small deletions.

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