Assembly of the terminal C5b-C9 complement components into the cytolytic C5b-9 complex is accompanied by exposure of characteristic neoantigens on the macromolecule. We report the production and characterization of mouse monoclonal antibodies to C9-dependent neoantigens of human C5b-9. Binding-inhibition assays with EDTA-human plasma and micro-ELISA assays with purified C9 showed that the antibodies did not react with native complement components and thus confirmed the specificity of the antibodies for the neoantigens. The monoclonal antibodies did, however, cross-react with cytolyticaIly inactive, fluid-phase C5b-9 complexes, Thus, expression of the neoantigenic determinants was not dependent on the formation of high molecular weight C9 polymers with the complex, since these are absent in fluid-phase C5b-9. Radioiodinated antibodies could be utilized in immunoradiometric assays for the detection and quantitation of C5b-9 on cell membranes. Cross-reactivities of the antibodies with C9-dependent neoantigens of several other animal species were examined and antibody clones cross-reacting with rabbit (clones 3BI, 3Dg, and 2F3), sheep (clones 3Dg and 2F3) and guinea-pig (clone 3D8) neoantigens were identified. Three of four tested clones (3D8, 2F3, IA12) precipitated C5b-9 complexes in double-diffusion assays, probably due to their interaction with multiple and repeating C9-epitopes on the terminal complexes. The monoclonal antibodies will be of value for definitive identification and quantitation of C5b-9 on cell membranes and in tissues, and for establishing immunoassays for detection and quantitation of terminal fluid-phase C5b-9 complexes in plasma.
Skip Nav Destination
Article navigation
Research Article|
August 01 1985
Monoclonal antibodies against neoantigens of the terminal C5b-9 complex of human complement
Ferdinand Hugo;
Ferdinand Hugo
1Institute of Medical Microbiology, University of Giessen, Schubertstrasse 1, D-6300 Giessen, German Federal Republic
Search for other works by this author on:
Dieter Jenne;
Dieter Jenne
1Institute of Medical Microbiology, University of Giessen, Schubertstrasse 1, D-6300 Giessen, German Federal Republic
Search for other works by this author on:
Sucharit Bhakdi
Sucharit Bhakdi
1Institute of Medical Microbiology, University of Giessen, Schubertstrasse 1, D-6300 Giessen, German Federal Republic
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
July 11 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (8): 649–658.
Article history
Received:
July 11 1985
Citation
Ferdinand Hugo, Dieter Jenne, Sucharit Bhakdi; Monoclonal antibodies against neoantigens of the terminal C5b-9 complex of human complement. Biosci Rep 1 August 1985; 5 (8): 649–658. doi: https://doi.org/10.1007/BF01116996
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |