Tyrosine phosphate is a frequently observed modification in a limited number of protein molecules, principally the products of viral oncogenes and membrane receptors for mitogenic growth factors. Such a group is therefore an attractive candidate for affinity selection of such phosphoproteins by antibodies with specificity for tyrosine phosphate. This report demonstrates that antisera raised against 4-aminobenzylphosphonic acid have specificity for tyrosine phosphate, but are not inhibited by tyrosine sulphate. This observation has important ramifications for the detection and isolation of phosphotyrosine-containing proteins without co-purification or detection of tyrosine sulphate-containing proteins, which are present in cells of all lineages. Furthermore, we demonstrate that antisera raised against the sulphanilate moiety are not reactive with tyrosine sulphate.
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August 01 1985
Rabbit antibodies with specificity for tyrosine phosphate are not reactive with tyrosine sulphate
Jane A. Jones;
Jane A. Jones
1Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, UK
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William Cushley
William Cushley
1Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ, UK
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Publisher: Portland Press Ltd
Received:
August 16 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (8): 707–714.
Article history
Received:
August 16 1985
Citation
Jane A. Jones, William Cushley; Rabbit antibodies with specificity for tyrosine phosphate are not reactive with tyrosine sulphate. Biosci Rep 1 August 1985; 5 (8): 707–714. doi: https://doi.org/10.1007/BF01117004
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