A monoclonal antibody (Mab J1-31) has been produced by using human brain homogenate as immunogen in mouse. Double-label immunofluorescence microscopy on cryostat sections of human, rabbit and rat brain, reveals staining of cells that are also stained with antiserum to glial fibrillary acidic protein (GFAP, a commonly used marker protein for astrocytes). However, there is no decrease in staining due to Mab J1-31 in sections incubated in antiserum to GFAP prior to incubation with the J1-31 ascites fluid. Immunoprecipitation of aqueous and detergent extracts of brain homogenate gives a single band at 30K by SDS PAGE followed by autoradiography. Immunoelectron microscopy shows that the J1-31 antigen is associated with the cytoskeleton. Thus, the Mab J1-31 recognizes a new protein present in GFAP positive cells (astrocytes) in the brain.
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January 01 1986
A new “marker” protein for astrocytes
Rajkumari Singh;
Rajkumari Singh
1Departments of Zoology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
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S. K. Malhotra
S. K. Malhotra
1Departments of Zoology and Immunology, University of Alberta, Edmonton, Alberta T6G 2E9, Canada
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Publisher: Portland Press Ltd
Received:
November 18 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (1): 73–80.
Article history
Received:
November 18 1985
Citation
Rajkumari Singh, Bhagirath Singh, S. K. Malhotra; A new “marker” protein for astrocytes. Biosci Rep 1 January 1986; 6 (1): 73–80. doi: https://doi.org/10.1007/BF01145181
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