Rat immunoglobulin (IgG) was covalently bound to N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers via glycylglycyl spacer. The resultant conjugate, free IgG and HPMA copolymer (containing a low percentage of tyrosinamide to facilitate radiolabelling) were radioiodinated, and their rates of pinocytic uptake, intracellular degradation and exocytic release by rat visceral yolk sacs cultured in vitro were determined. Free IgG was pinocytosed rapidly by the yolk sac and some IgG was subject to intracellular proteolysis. In comparison the IgG-HPMA copolymer conjugate was captured more slowly, but faster than unmodified HPMA. IgG was also exocytosed rapidly by the yolk sac following pinocytic capture and similarly IgG-HPMA copolymer had a much higher rate of release than unmodified H PMA. Measurement of tissue accumulation of125I-labelled IgG-H PMA copolymer in the presence of increasing concentrations of non-radiolabelled IgG showed competition for membrane binding sites between the free, and polymer-bound immunoglobulin. These experiments indicate that immunoglobulins can be covalently bound to a soluble polymer developed as a drug-carrier in such a way that they can still interact with specific membrane receptors and they are subsequently subjected to specific cellular transport mechanisms.
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October 01 1986
Pinocytic capture and exocytosis of rat immunoglobulin IgG-N-(2-hydroxy-propyl)methacrylamide copolymer conjugates by rat visceral yolk sacs cultured in vitro
Ruth Duncan;
Ruth Duncan
1Department of Biological Sciences, University of Keele, Keele, Staffordshire, ST5 5BG, England
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Hazel C. Cable;
Hazel C. Cable
1Department of Biological Sciences, University of Keele, Keele, Staffordshire, ST5 5BG, England
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Jiri Strohalm;
Jiri Strohalm
1Department of Biological Sciences, University of Keele, Keele, Staffordshire, ST5 5BG, England
1Institute of Macromolecular Chemistry, Czechoslovak Academy of Sciences, 162 06 Prague 6, Czechoslovakia
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Jindřich Kopeček
Jindřich Kopeček
1Department of Biological Sciences, University of Keele, Keele, Staffordshire, ST5 5BG, England
1Institute of Macromolecular Chemistry, Czechoslovak Academy of Sciences, 162 06 Prague 6, Czechoslovakia
2Department of Bioengineering and Pharmaceutics, University of Utah, 2059 Merrill Engineering Building, Salt Lake City, Utah 84112, USA
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Publisher: Portland Press Ltd
Received:
October 21 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (10): 869–877.
Article history
Received:
October 21 1986
Citation
Ruth Duncan, Hazel C. Cable, Jiri Strohalm, Jindřich Kopeček; Pinocytic capture and exocytosis of rat immunoglobulin IgG-N-(2-hydroxy-propyl)methacrylamide copolymer conjugates by rat visceral yolk sacs cultured in vitro. Biosci Rep 1 October 1986; 6 (10): 869–877. doi: https://doi.org/10.1007/BF01116240
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