Alpha-sarcin and gelonin, two proteins which inactivate the 60S ribosomal subunit, interact with Cibacron blue and bind to blue dextran-Sepharose, from which they are partially desorbed by nucleoside triphosphates and, more efficiently, by homopolynucleotides. It is further shown that the two proteins bind to poly(U)-Sepharose and that homopolynucleotides protect dilute solutions of both inhibitors from inactivation. It is suggested that the presence of a polynucleotide site on alphasarcin is related to its ribonuclease activity. The existence of a similar site on gelonin might be a clue to its yet unknown mechanism of action.
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Rapid Communication| October 01 1986
Interaction of alpha-sarcin and gelonin with Cibacron blue
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Simonetta Sperti, Lucio Montanaro, Fioretta Rambelli, Mariacristina Zamboni; Interaction of alpha-sarcin and gelonin with Cibacron blue. Biosci Rep 1 October 1986; 6 (10): 901–908. doi: https://doi.org/10.1007/BF01116244
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