Experiments are described which probe the relationship between three sequence elements which make up the eukaryotic RNA polymerase II promoter. A cloned eukaryotic gene, from which the TATA-box and 400 base pairs of Y-flanking sequence has been deleted, is still transcriptionally active in vivo (following its transfection into cultured mammalian cells) and in vitro. Deletion has appropriately positioned a cluster of five TATA box-like sequences upstream from multiple potential cap sites. Which cap sites are actually used can be predicted from the DNA sequence of TATA box-like sequences and their spatial relationship with respect to possible transcriptional start sites, although there appears to be some difference in cap site utilisation in vitro and in vivo. Data suggest that deletion has also removed “upstream” sequences which affect promoter function.
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November 01 1986
DNA sequences which influence the selection and efficient utilisation of transcriptional start sites of a eukaryotic gene by RNA polymerase II in vitro and in vivo
Balazs J. Kovacs;
Balazs J. Kovacs
1Department of Biochemistry, University College London, Gower Street, London WC1E 6BT, UK
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Peter H. W. Butterworth
Peter H. W. Butterworth
1Department of Biochemistry, University College London, Gower Street, London WC1E 6BT, UK
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Publisher: Portland Press Ltd
Received:
November 21 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (11): 937–944.
Article history
Received:
November 21 1986
Citation
Balazs J. Kovacs, Peter H. W. Butterworth; DNA sequences which influence the selection and efficient utilisation of transcriptional start sites of a eukaryotic gene by RNA polymerase II in vitro and in vivo. Biosci Rep 1 November 1986; 6 (11): 937–944. doi: https://doi.org/10.1007/BF01114969
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