A rapid and simple method for the isolation of the two chains of ricin is described. The method involves two chromatographic runs, the first on blue-Sepharose and the second on blue dextran-Sepharose. It is moreover shown that the A chain of ricin, but not the B chain, binds to poly(U)-Sepharose.
Rapid Communication| December 01 1986
Dye affinity chromatography of ricin subunits
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Simonetta Sperti, Lucio Montanaro, Fioretta Rambelli; Dye affinity chromatography of ricin subunits. Biosci Rep 1 December 1986; 6 (12): 1035–1040. doi: https://doi.org/10.1007/BF01141024
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