A simple method for evaluating leukocyte locomotion in vitro has been developed and validated for several chemoattractants. The multiwell cap assay (MWCA) comprises chambers constructed from readily available disposable plastics and is quickly assembled, permitting large experimental protocols. Leukocytes which have migrated through a micropore filter are recovered and counted electronically yielding a precise, objective result. Coefficients of variation are approximately 6%.
Multiwell cap assay: A simple objective method for the assessment of leukocyte locomotion in vitro
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Anne M. Evans, J. David Chambers, John R. Hobbs; Multiwell cap assay: A simple objective method for the assessment of leukocyte locomotion in vitro. Biosci Rep 1 December 1986; 6 (12): 1041–1047. doi: https://doi.org/10.1007/BF01141025
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