A two-dimensional projection map was computed of the Ca2+-ATPase molecules in sarcoplasmic reticulum, isolated from rabbit skeletal muscle. Crystalline arrays of Ca2+-ATPase molecules were formed by incubating the membrane vesicles with phospholipase A2 and dialysing against Tris/HCl buffer. Ca2+-ATPase molecules appear as quasi-triangular blobs in the projection map and seem to form dimers. The projection map seems to indicate an enzyme conformation somewhat similar to vanadate-induced crystals but different from lanthanide-induced crystals of Ca2*-ATPase.
Rapid Communication| December 01 1986
Two-dimensional structure of phospholipase induced crystals of Ca2+-ATPase from sarcoplasmic reticulum
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M. Misra, S. K. Malhotra; Two-dimensional structure of phospholipase induced crystals of Ca2+-ATPase from sarcoplasmic reticulum. Biosci Rep 1 December 1986; 6 (12): 1065–1070. doi: https://doi.org/10.1007/BF01141028
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