In Neurospora crassa, there is a single pyruvate kinase (PK) consisting of four identical subunits of ∼60k daltons. Northern and dot blot hybridization studies, using most of the yeast pyruvate kinase gene as a probe, suggest the presence of two distinct mRNA species for pyruvate kinase, separable on the basis of the length of their polyadenylated tails, by oligo(dT)cellulose chromatography. These messages are present in polysomes, immuno-precipitated by anti-PK antibodies, indicating probable translation in vivo. Fractions containing both messages were translated in vitro in the heterologous systems as well as in a homologous N. crassa lysate, the newly-synthesized PK being detected by immunoadsorption. Protection studies using S1-nuclease suggest no major structural differences in the 5′-untranslated and most of the coding regions of the two messages.
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February 01 1986
A study of the messenger RNA encoding pyruvate kinase of Neurospora crassa
M. Devchand;
M. Devchand
1Department of Biology, University of Calgary, Calgary, Alberta, T2N 1N4
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M. Kapoor
M. Kapoor
1Department of Biology, University of Calgary, Calgary, Alberta, T2N 1N4
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Publisher: Portland Press Ltd
Received:
December 23 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (2): 201–208.
Article history
Received:
December 23 1985
Citation
M. Devchand, M. Kapoor; A study of the messenger RNA encoding pyruvate kinase of Neurospora crassa. Biosci Rep 1 February 1986; 6 (2): 201–208. doi: https://doi.org/10.1007/BF01115007
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