Six groups of 5 male rats (starting body weight 109 g) were allowed free access to a conventional rat diet. At 4 hourly intervals, starting at 10.00 h muscle protein synthesis was measured. By relating the weights of the gastrocnemius and soleus muscles to the initial body weights of the animals (i.e., at 09.30, day 1), a linear increase in muscle weight throughout the day was demonstrated. The fractional rate of muscle protein synthesis varied from 16.8% per day to 20.3% per day in gastrocnemius muscle and from 17.9% per day and 22.1% per day in the soleus. It was calculated that the maximum error incurred in estimating daily muscle protein synthesis by extrapolation of the value at any one time was 6% in gastrocnemius and 9% in soleus. It is concluded that calculations of the average rate of muscle protein degradation based on the difference between the rates of synthesis and deposition are generally valid in rats allowed free access to an adequate diet.
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February 01 1986
Protein synthesis in skeletal muscle measured at different times during a 24 hour period
P. J. Reeds;
P. J. Reeds
1Biochemistry Division, Rowett Research Institute, Bucksburn, Aberdeen, Scotland
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R. M. Palmer;
R. M. Palmer
1Biochemistry Division, Rowett Research Institute, Bucksburn, Aberdeen, Scotland
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S. M. Hay;
S. M. Hay
1Biochemistry Division, Rowett Research Institute, Bucksburn, Aberdeen, Scotland
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D. N. McMillan
D. N. McMillan
1Biochemistry Division, Rowett Research Institute, Bucksburn, Aberdeen, Scotland
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Publisher: Portland Press Ltd
Received:
January 16 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (2): 209–213.
Article history
Received:
January 16 1986
Citation
P. J. Reeds, R. M. Palmer, S. M. Hay, D. N. McMillan; Protein synthesis in skeletal muscle measured at different times during a 24 hour period. Biosci Rep 1 February 1986; 6 (2): 209–213. doi: https://doi.org/10.1007/BF01115008
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