Incubation of mouse thymocytes with 10μM monensin for 1 hour induces morphological alterations characterized by the extensive dilatation and vacuolization of the Golgi complex. This effect is used to study the transport and utilization of labelled sugar nucleotides into intracellular vesicles by using thymocytes whose plasma membrane has been permeabilized by ammonium chloride treatment. It is demonstrated that monensin stimulates the incorporation of labelled sialyl, fucosyl, galactosyl, and N-acetylglucosaminyl residues. This enhanced incorporation is not due to a direct effect of monensin on glycosyltransferase activities themselves but is a consequence of a higher entry and accumulation of labelled sugar nucleotides in the dilated vesicles.
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February 01 1986
Dilatation of Golgi vesicles by monensin leads to enhanced accumulation of sugar nucleotides
Roméo Cecchelli;
Roméo Cecchelli
1Laboratoire de Chimie Biologique and Laboratoire Associé au CNRS no. 217.
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René Cacan;
René Cacan
1Laboratoire de Chimie Biologique and Laboratoire Associé au CNRS no. 217.
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Eliane Porchet-Hennere;
Eliane Porchet-Hennere
2Laboratoire Associé au CNRS no. 148, Université des Sciences et Techniques de Lille, 59655 Villeneuve d'Ascq Cédex, France
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André Verbert
André Verbert
1Laboratoire de Chimie Biologique and Laboratoire Associé au CNRS no. 217.
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Publisher: Portland Press Ltd
Received:
January 17 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (2): 227–234.
Article history
Received:
January 17 1986
Citation
Roméo Cecchelli, René Cacan, Eliane Porchet-Hennere, André Verbert; Dilatation of Golgi vesicles by monensin leads to enhanced accumulation of sugar nucleotides. Biosci Rep 1 February 1986; 6 (2): 227–234. doi: https://doi.org/10.1007/BF01115011
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