We have made simultaneous spectrophotometric and O2 measurements on suspensions of rat neutrophils during activation of the respiratory burst. Under aerobic conditions an absorption increase attributable to myeloperoxidase compound II was observed in parallel with the rapid phase of O2 uptake. Identification of this compound was confirmed by analysis of a spectrum obtained with purified myeloperoxidase and H2O2. Whereas a second addition of stimulus did not increase O2 uptake any further, a second phase of myeloperoxidase release and compound II formation was observed. These results suggest that in vivo myeloperoxidase reacts with H2O2 generated via the respiratory burst to form compound II under conditions in which the chlorination reaction would be the expected major pathway.
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March 01 1986
Formation of myeloperoxidase compound II during aerobic stimulation of rat neutrophils
Steven W. Edwards;
Steven W. Edwards
1Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX
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David Lloyd
David Lloyd
2Department of Microbiology, University College, Newport Road, Cardiff CF2 1TA, UK
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Publisher: Portland Press Ltd
Received:
January 21 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (3): 275–282.
Article history
Received:
January 21 1986
Citation
Steven W. Edwards, David Lloyd; Formation of myeloperoxidase compound II during aerobic stimulation of rat neutrophils. Biosci Rep 1 March 1986; 6 (3): 275–282. doi: https://doi.org/10.1007/BF01115156
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