The binding of somatostatin was studied in the cytosolic fraction of bovine gallbladder mucosa. The binding reaction depended on time, temperature and pH, and was reversible, saturable and specific. Stoichiometric data suggested the presence of two classes of binding sites: a class with high affinity (Kd=23.6 n M) and low capacity (3.7 pmol somatostatin/mg protein) and a class with low affinity (Kd=284.6 n M) and high capacity (85.0 pmol somatostatin/mg protein) at 37°C and pH 7.4. The binding sites were highly specific for somatostatin since peptides such as [Leu]enkephalin, neurotensin, vasoactive intestinal peptide and substance P showed practically no effect upon somatostatin binding. The presence of somatostatin-binding sites in the cytosolic fraction of gallbladder mucosa, together with the known occurrence of somatostatin nerve endings in the gallbladder strongly suggests that this peptide may be involved in the physiology and physiopathology of gallbladder mucosa.
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March 01 1986
Specific somatostatin-binding to cytosol of bovine gallbladder mucosa
E. Arilla;
E. Arilla
1Departamento de Bioquímica, Facultad de Medicina, Universidad de Alcala de Henares, Madrid, Spain
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B. Colás;
B. Colás
1Departamento de Bioquímica, Facultad de Medicina, Universidad de Alcala de Henares, Madrid, Spain
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J. C. Prieto
J. C. Prieto
1Departamento de Bioquímica, Facultad de Medicina, Universidad de Alcala de Henares, Madrid, Spain
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Publisher: Portland Press Ltd
Received:
January 23 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (3): 283–291.
Article history
Received:
January 23 1986
Citation
E. Arilla, B. Colás, J. C. Prieto; Specific somatostatin-binding to cytosol of bovine gallbladder mucosa. Biosci Rep 1 March 1986; 6 (3): 283–291. doi: https://doi.org/10.1007/BF01115157
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