The effects of D-glucose, D-glyceraldehyde, glibenclamide, D-600, NH4+ and high concentrations of K+ on cytoplasmic pH (pHi) were investigated in dispersed and cultured pancreatic β-cells from ob/ob mice. The cytoplasmic pH was measured with the fluorescent H+-indicator quene 1. The average pHi value in resting β-cells was 6.71. Addition of 20 m M of the physiological stimulus D-glucose increased pHi with 0.05 units. Both glibenclamide and high concentrations of K+ decreased pHi. The latter effects were completely reversed by D-600, supporting the notion that free cytoplasmic Ca2+ can be involved in the regulation of pHi. In contrast to D-glucose, 10m M of D-glyceraldehyde decreased pHi by 0.09 units, an effect persisting even in the presence of D-600. From the present study it is evident that D-glyceraldehyde and D-glucose have opposite effects on pHi in pancreatic β-cells.
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April 01 1986
Direct evidence for opposite effects of D-glucose and D-glyceraldehyde on cytoplasmic pH of mouse pancreatic β-cells
Per Arkhammar;
Per Arkhammar
1Department of Medical Cell Biology, Biomedicum, University of Uppsala, S-751 23 Uppsala, Sweden
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Per-Olof Berggren;
Per-Olof Berggren
1Department of Medical Cell Biology, Biomedicum, University of Uppsala, S-751 23 Uppsala, Sweden
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Publisher: Portland Press Ltd
Received:
February 05 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (4): 355–361.
Article history
Received:
February 05 1986
Citation
Per Arkhammar, Per-Olof Berggren, Patrik Rorsman; Direct evidence for opposite effects of D-glucose and D-glyceraldehyde on cytoplasmic pH of mouse pancreatic β-cells. Biosci Rep 1 April 1986; 6 (4): 355–361. doi: https://doi.org/10.1007/BF01116422
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