Various constructions of human haptoglobin (Hp) cDNA coding either for the complete α2FSβ precursor protein or only for the β subunit have been placed under the control of the λPR promoter in the bacterial expression vector pCQV2 (Queen, 1983). In addition to the expected 45,000 dalton polypeptide synthesized after induction of the PR promoter, the complete α2FSβ constructions constitutively express a smaller polypeptide of ∼30,000 dalton corresponding to a truncated Hp protein. Computer analysis of the HpcDNA revealed the presence of two strong potential bacterial promoters (α2PF and α2PS) located in the duplicated α2FS sequence. Both Hp promoter signals are followed by potential mRNA start sites and ribosome binding sites at a compatible distance from initiation codons. In addition, the Hpα2 cDNA sequence, when fused upstream to the cDNA coding for α1-antitrypsin, constitutively promotes in vivo the efficient expression of an hybrid protein specifically recognized by antibodies raised against α1-antitrypsin or haptoglobin.
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April 01 1986
The α2 cDNA sequence of human haptoglobin carries a bacterial promoter functional in vivo
Ariane van der Straten;
Ariane van der Straten
1Service for Applied Genetics, University of Brussels, rue de l'Industrie 24, B-1400 Nivelles, Belgium
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Rosette Loriau;
Rosette Loriau
1Service for Applied Genetics, University of Brussels, rue de l'Industrie 24, B-1400 Nivelles, Belgium
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Albert Herzog;
Albert Herzog
1Service for Applied Genetics, University of Brussels, rue de l'Industrie 24, B-1400 Nivelles, Belgium
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Alex Bollen
Alex Bollen
1Service for Applied Genetics, University of Brussels, rue de l'Industrie 24, B-1400 Nivelles, Belgium
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Publisher: Portland Press Ltd
Received:
February 06 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (4): 363–373.
Article history
Received:
February 06 1986
Citation
Ariane van der Straten, Rosette Loriau, Albert Herzog, Alex Bollen; The α2 cDNA sequence of human haptoglobin carries a bacterial promoter functional in vivo. Biosci Rep 1 April 1986; 6 (4): 363–373. doi: https://doi.org/10.1007/BF01116423
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