In order to provide a sensitive and quantitative detection method of ras p21 at the cytological level, the monoclonal antibody Y 13 259 and iodinated protein A were used to locate the ras protein in various mammalian cell lines. The subsequent autoradiograph can be analysed by a computer-assisted system which showed in these reported experiments that the relative levels of p21 detected in these cells corresponded to results obtained earlier using conventional biochemical methods.
A radioimmunocytological quantitative method for the rapid detection of ras oncogene p21 protein in mammalian cells
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Demetrios A. Spandidos, Todor Dimitrov, Ephraim Y. Kam; A radioimmunocytological quantitative method for the rapid detection of ras oncogene p21 protein in mammalian cells. Biosci Rep 1 April 1986; 6 (4): 375–379. doi: https://doi.org/10.1007/BF01116424
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