We identified fibrils from non-transmissible systemic and cerebral amyloidosis using the purification method of scrapie-associated fibrils (SAF). The fibrils possessed the same nature of congophilia, filamentous structures and molecular weights as amyloid fibrils, and were resistant to Proteinase K digestion. This SAF method makes for a rapid extraction from amyloid-laden tissues. The method, therefore, may purify nontransmissible amyloids alone or together with SAF proteins.
Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis
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Tetsuyuki Kitamoto, Kohtaro Hikita, Takatoshi Tashima, Jun Tateishi, Yuji Sato; Scrapie-associated fibrils (SAF) purification method yields amyloid proteins from systemic and cerebral amyloidosis. Biosci Rep 1 May 1986; 6 (5): 459–465. doi: https://doi.org/10.1007/BF01116137
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