Nucleosomal core particles containing the right- and left-handed conformations of DNA were examined for their ability to support the B→Z or Z→B transition. Nucleosomes were assembled onto the B- and Z-conformations of poly[d(Gm5C)] and the B-conformation of poly[d(GC)] as previously described (1). Absorbance and circular dichroic spectroscopy indicated that the DNA on all three core particle populations could undergo the conformational B↔Z transition. Further, the right- to left-handed transition for both poly[d(Gm5C)] and poly[d(GC)] appeared to be facilitated by the DNAs association with the histone octamer. The DNA remained associated with the protein core subsequent to the transition, and electron microscopy and sedimentation velocity analysis indicated that there were no gross changes in nucleosomal structure. However, a change in the sedimentation value of the poly[d(Gm5C)] core particles was detected when the conformation of the DNA was altered from B to Z, resulting in a lower S20,w value for the Z-form particles than for the corresponding B-form particles.
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May 01 1986
Assembly of DNA onto the histone octamer facilitates the B-to-Z transition
F. D. Miller;
F. D. Miller
1Department of Medical Biochemistry, The University of Calgary, Calgary, Alberta, Canada T2N 4N1
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J. B. Rattner;
J. B. Rattner
2Department of Anatomy, The University of Calgary, Calgary, Alberta, Canada T2N 4N1
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J. H. van de Sande
J. H. van de Sande
1Department of Medical Biochemistry, The University of Calgary, Calgary, Alberta, Canada T2N 4N1
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Publisher: Portland Press Ltd
Received:
March 25 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (5): 467–476.
Article history
Received:
March 25 1986
Citation
F. D. Miller, J. B. Rattner, J. H. van de Sande; Assembly of DNA onto the histone octamer facilitates the B-to-Z transition. Biosci Rep 1 May 1986; 6 (5): 467–476. doi: https://doi.org/10.1007/BF01116138
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