Compounds containing the −PO3H2 function, such as monoesters of phosphoric acid and phosphonic acids, specifically bind to aluminium oxide in aqueous solution under experimental conditions where non-phosphorylated compounds are completely desorbed. The bound organic phosphate can be specifically displaced by aqueous solution of inorganic phosphates thus allowing their separation or detection by a technique similar to that of affinity chromatography. The consequences of this finding for phosphate compound biochemistry are discussed.
Selective retention of organic phosphate esters and phosphonates on aluminium oxide
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M-A. Coletti-Previero, M. Pugnière, H. Mattras, J. C. Nicolas, A. Previero; Selective retention of organic phosphate esters and phosphonates on aluminium oxide. Biosci Rep 1 May 1986; 6 (5): 477–483. doi: https://doi.org/10.1007/BF01116139
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