Bovine t hymic peptide extract (1–100 μg/ml) is shown to completely inhibit the binding of [125I]VIP to rat blood mononuclear cells, lymphoid cells of spleen, and liver plasma membranes. In the three models, the bovine thymic peptide extract inhibits [125I]VIP binding with a potency that is 4000–7000 times lower than that of the native VIP, on a weight basis. In rat liver plasma membranes, the bovine thymic peptide extract stimulates adenylate cyclase with a maximal efficiency that is similar to that of VIP. At maximal doses, VIP and thymic peptide extract do not exert an additive effect on adenylate cyclase, suggesting that the activation of the enzyme by the bovine thymic peptide extract occurs through VIP receptors. Finally, no VIP-like immunoreactivity was detected in the thymic peptide extract using an antiserum raised against mammalian VIP. All these data suggest the presence in the bovine thymic peptide extract of a new substance which behaves as a VIP agonist in rat.
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June 01 1986
Interaction of a bovine thymic peptide extract with vasoactive intestinal peptide (VIP) receptors
J. M. Guerrero;
J. M. Guerrero
1Dept of Biochemistry, School of Medicine, University of Seville, Avda. Sánchez Pizjuán, 4, 41009-Seville, Spain
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R. Goberna;
R. Goberna
1Dept of Biochemistry, School of Medicine, University of Seville, Avda. Sánchez Pizjuán, 4, 41009-Seville, Spain
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P. Molinero;
P. Molinero
1Dept of Biochemistry, School of Medicine, University of Seville, Avda. Sánchez Pizjuán, 4, 41009-Seville, Spain
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J. Jimenez;
J. Jimenez
1Dept of Biochemistry, School of Medicine, University of Seville, Avda. Sánchez Pizjuán, 4, 41009-Seville, Spain
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J. R. Calvo
J. R. Calvo
1Dept of Biochemistry, School of Medicine, University of Seville, Avda. Sánchez Pizjuán, 4, 41009-Seville, Spain
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Publisher: Portland Press Ltd
Received:
June 19 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (6): 579–584.
Article history
Received:
June 19 1986
Citation
J. M. Guerrero, R. Goberna, P. Molinero, J. Jimenez, J. R. Calvo; Interaction of a bovine thymic peptide extract with vasoactive intestinal peptide (VIP) receptors. Biosci Rep 1 June 1986; 6 (6): 579–584. doi: https://doi.org/10.1007/BF01114955
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