Protamine-like sequences have been identified in poly A(+) mRNAs from mammalian testes by the use of a common, complementary oligonucleotide (GCAGCANCK PTANCKNGCCAT; predicted from the common N-terminal amino acid sequence, MARYRCC, seen in several mammalian P1 protamines [D. J. McKay, B. S. Renaux and G. H. Dixon, Bioscience Reports5:383–391 (1985)]). This oligonucleotide was utilized to prepare species-specific, primer-extended transcripts for use as Northern blotting probes. Analysis of the mRNA primer-extended transcripts revealed a discrete and similar set of products common to both bull and rat testis mRNAs which were distinct from those obtained from human testis mRNA. Northern analysis of total poly A(+) mRNAs from the corresponding mammalian testis was consistent with these results and suggests that bull and rat protamine mRNAs are more closely related to each other than to human protamine mRNA.
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June 01 1986
Oligonucleotides as probes for mammalian protamine mRNAs
Stephen A. Krawetz;
Stephen A. Krawetz
1Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Health Sciences Centre, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1
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Wayne Connor;
Wayne Connor
1Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Health Sciences Centre, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1
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Gordon H. Dixon
Gordon H. Dixon
1Department of Medical Biochemistry, Faculty of Medicine, University of Calgary, Health Sciences Centre, 3330 Hospital Drive N.W., Calgary, Alberta, Canada T2N 4N1
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Publisher: Portland Press Ltd
Received:
June 20 1986
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1986 Plenum Publishing Corporation
1986
Biosci Rep (1986) 6 (6): 585–590.
Article history
Received:
June 20 1986
Citation
Stephen A. Krawetz, Wayne Connor, Gordon H. Dixon; Oligonucleotides as probes for mammalian protamine mRNAs. Biosci Rep 1 June 1986; 6 (6): 585–590. doi: https://doi.org/10.1007/BF01114956
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