In the present paper we discuss the properties of a recently developed preparation of isolated neurosecretory nerve endings obtained from the rate neurohypophysis. These nerve terminals release two neurohormones, oxytocin and vasopressin, which are easily assayed by radioimmunoassay. Depolarization-induced secretion is dependent on the same parameters as those regulating release from the whole neural lobe. The isolated nerve endings can be permeabilized by means of digitonin; a treatment which gives direct access to the cytoplasm allowing the study of the minimal requirements for inducing neuropeptide release. Furthermore, some nerve endings are large enough to allow the use of the patch-clamp technique. In the present paper we present evidences which show that the isolated neurohypophysial nerve terminals represent a protent tool for studying the mechanism of stimulus-secretion.
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May 01 1987
Isolated neurosecretory nerve endings as a tool for studying the mechanism of stimulus-secretion coupling
Jean J. Nordmann;
Jean J. Nordmann
1Centre de Neurochimie, 5, rue Blaise Pascal, 67084 Strasbourg, France
1Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545, USA
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Govindan Dayanithi;
Govindan Dayanithi
1Centre de Neurochimie, 5, rue Blaise Pascal, 67084 Strasbourg, France
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José R. Lemos
José R. Lemos
1Centre de Neurochimie, 5, rue Blaise Pascal, 67084 Strasbourg, France
1Worcester Foundation for Experimental Biology, Shrewsbury, MA 01545, USA
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Publisher: Portland Press Ltd
Received:
May 06 1987
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1987 Plenum Publishing Corporation
1987
Biosci Rep (1987) 7 (5): 411–426.
Article history
Received:
May 06 1987
Citation
Jean J. Nordmann, Govindan Dayanithi, José R. Lemos; Isolated neurosecretory nerve endings as a tool for studying the mechanism of stimulus-secretion coupling. Biosci Rep 1 May 1987; 7 (5): 411–426. doi: https://doi.org/10.1007/BF01362504
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