The rapid increase in protein synthesis that occurs on addition of insulin (1 mU/ml) to stepped-down 3T3 cells was blocked by pre-incubation of the cells with pertussis toxin. Cholera toxin on the other hand stimulated protein synthesis and this effect was insensitive to actinomycin D and inhibited by pro-treatment of the cells with phorbol dibutyrate to deplete cell protein kinase C. Insulin was found to cause a rapid and transient increase in diacylglycerol (DAG) synthesis. The insulin-induced increase in diacylglycerol was blocked by pertussis toxin. Exogenous DAG (10 μM) stimulated protein synthesis within 1 hour. The results suggest that insuIin stimulates ribosomal activity through a signal mechanism that involves a G-protein mediated activation of phospholipase C to increase DAG levels.
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July 01 1987
Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts: Evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism Available to Purchase
John E. Hesketh;
John E. Hesketh
1The Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB2 9SB
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Gillian P. Campbell
Gillian P. Campbell
1The Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB2 9SB
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Publisher: Portland Press Ltd
Received:
June 12 1987
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1987 Plenum Publishing Corporation
1987
Biosci Rep (1987) 7 (7): 533–541.
Article history
Received:
June 12 1987
Citation
John E. Hesketh, Gillian P. Campbell; Effects of insulin, pertussis toxin and cholera toxin on protein synthesis and diacylglycerol production in 3T3 fibroblasts: Evidence for a G-protein mediated activation of phospholipase C in the insulin signal mechanism. Biosci Rep 1 July 1987; 7 (7): 533–541. doi: https://doi.org/10.1007/BF01119769
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