Rat liver dolichol and dolichyl-P were labeled by injection of [3H]mevalonate into the portal vein and their rates of synthesis and breakdown determined. In the initial phase the radioactivity appeared in α-unsaturated polyprenols. Subsequent saturation required 90 min. The half-lives of dolichols in microsomes were between 80 and 118 h, and shorter dolichols had shorter values of T1/2. The half-lives of dolichols in lysosomes were between 115 and 137 h, while microsomal dolichyl-P exhibited a T1/2 of 32 h. Injected dolichol was recovered in the lysomes of hepatocytes and exhibited a rate of breakdown which was slower than that of the endogenous compound. These results indicate differences in the catabolism of dolichol at different subcellular locations, as well as differences between the catabolism of dolichol and dolichyl-P.
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April 01 1988
The half-lives of dolichol and dolichyl phosphate in rat liver
Conny Edlund;
Conny Edlund
1Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Sweden
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Ulf Brunk;
Ulf Brunk
3Department of Pathology, University of Linköping, Linköping, Sweden
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Tadeusz Chojnacki;
Tadeusz Chojnacki
4Institute of Biochemistry, Polish Academy of Sciences, Warsaw, Poland
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Gustav Dallner
Gustav Dallner
1Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, Sweden
2Department of Pathology at Huddinge Hospital, Karolinska Institutet, Stockholm, Sweden
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Publisher: Portland Press Ltd
Received:
February 01 1988
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1988 Plenum Publishing Corporation
1988
Biosci Rep (1988) 8 (2): 139–146.
Article history
Received:
February 01 1988
Citation
Conny Edlund, Ulf Brunk, Tadeusz Chojnacki, Gustav Dallner; The half-lives of dolichol and dolichyl phosphate in rat liver. Biosci Rep 1 April 1988; 8 (2): 139–146. doi: https://doi.org/10.1007/BF01116458
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