Total RNA from post mortem human caudate nucleus, cerebellum, cerebral cortex and pheochromocytoma tissues has been prepared. Northern blot analysis, using a single-stranded human proenkephalin A antisense probe (cRNA), revealed the existence of two different proenkephalin A-like sequences in the human caudate nucleus and pheochromocytoma RNA extracts of approximately 1400 and 1000 nucleotides in length respectively, whereas no specific RNA bands could be detected in the cortex and only the 1400 nucleotide band was present in the cerebellum. Under highly stringent hybridization conditions, the proenkephalin A-like RNA bands still appear, indicating that the detected RNA species have either identical or a closely related sequence to that of the wellcharacterized human proenkephalin A mRNA sequence.
Skip Nav Destination
Article navigation
Rapid Communication|
June 01 1988
A highly sensitive northern blot assay detects multiple proenkephalin a-like mRNAs in human caudate nucleus and pheochromocytoma
Hans-Jürg Monstein;
Hans-Jürg Monstein
1Department of Microbiology, Biotechnology Group, Swedish University of Agricultural Sciences, Box 7025, S-750 07 UPPSALA, Sweden
2Department of Pharmacology, Uppsala University, BMC, Box 591, S-751 24 Uppsala, Sweden
Search for other works by this author on:
Thomas Geijer
Thomas Geijer
2Department of Pharmacology, Uppsala University, BMC, Box 591, S-751 24 Uppsala, Sweden
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
March 01 1988
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1988 Plenum Publishing Corporation
1988
Biosci Rep (1988) 8 (3): 255–261.
Article history
Received:
March 01 1988
Citation
Hans-Jürg Monstein, Thomas Geijer; A highly sensitive northern blot assay detects multiple proenkephalin a-like mRNAs in human caudate nucleus and pheochromocytoma. Biosci Rep 1 June 1988; 8 (3): 255–261. doi: https://doi.org/10.1007/BF01115042
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |