Digestion of nuclear DNAs of five plants, namely Cucurbita maxima (red gourd), Trichosanthes anguina (snake gourd), Cucumis sativus (cucumber), Cajanus cajan (pigeon pea) and Phaseolus vulgaris (french bean) with the restriction endonuclease MboI yielded discrete size classes with molecular weights in the range of 0.5 to 5 kbp. The MboI digestion pattern of Cot 0.1 DNA in french bean is comparable with that of total DNA, indicating that these bands represented highly repeated DNA sequences. Cleavage of the DNAs with varying amounts of MboI indicated the dispersed nature of the repeat families. Southern hybridization studies using french bean highly repetitive DNA as a probe indicated more homology with repeats of pigeon pea and less homology with red gourd, snake gourd and cucumber repeats.
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October 01 1988
Identification of a dispersed MboI repeat family in five higher plant genomes
S. A. Ranade;
S. A. Ranade
1Division of Biochemical Sciences, National Chemical, Laboratory, Pune-411 008, India
1Biotechnology Group, Tata Energy Research Institute, 90 Jor Bagh, New Delhi-110 003, India
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M. D. Lagu;
M. D. Lagu
1Division of Biochemical Sciences, National Chemical, Laboratory, Pune-411 008, India
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S. M. Patankar;
S. M. Patankar
1Division of Biochemical Sciences, National Chemical, Laboratory, Pune-411 008, India
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M. M. Dabak;
M. M. Dabak
1Division of Biochemical Sciences, National Chemical, Laboratory, Pune-411 008, India
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M. S. Dhar;
M. S. Dhar
1Division of Biochemical Sciences, National Chemical, Laboratory, Pune-411 008, India
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V. S. Gupta;
V. S. Gupta
1Division of Biochemical Sciences, National Chemical, Laboratory, Pune-411 008, India
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P. K. Ranjekar
P. K. Ranjekar
1Division of Biochemical Sciences, National Chemical, Laboratory, Pune-411 008, India
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Publisher: Portland Press Ltd
Received:
December 15 1987
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1988 Plenum Publishing Corporation
1988
Biosci Rep (1988) 8 (5): 435–441.
Article history
Received:
December 15 1987
Citation
S. A. Ranade, M. D. Lagu, S. M. Patankar, M. M. Dabak, M. S. Dhar, V. S. Gupta, P. K. Ranjekar; Identification of a dispersed MboI repeat family in five higher plant genomes. Biosci Rep 1 October 1988; 8 (5): 435–441. doi: https://doi.org/10.1007/BF01121641
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