ATP is not required for exocytosis from permeabilised mast cells, and therefore there is no direct role for protein phosphorylation in the late stages of the activation pathway. We have measured the timecourse of exocytosis from permeabilised cells triggered to release hexosaminidase following addition of Ca2+ to cells equilibrated for 2 min with GTP-γ-S. If ATP is included at the time of permeabilisation, then exocytosis commences after a delay, the duration of which depends on the square root of the product [Ca2+][GTP-γ-S], and which may extend to beyond 3 min. When ATP is excluded then the maximal rate of exocytosis is established within 3 secs of completing the effector combination. These results suggest that the achievement of a new steady-state, induced by Ca2+ and GTP-γ-S, and required for exocytosis is inhibited by ATP. From this we conclude that dephosphorylation of an unknown regulator protein may comprise a step in the exocytotic pathway.
Skip Nav Destination
Article navigation
Rapid Communication|
February 01 1989
ATP inhibits onset of exocytosis in permeabilised mast cells
P. E. R. Tatham;
P. E. R. Tatham
1Department of Experimental Pathology, University College London, University Street, London WC1E 6JJ, UK
Search for other works by this author on:
B. D. Gomperts
B. D. Gomperts
1Department of Experimental Pathology, University College London, University Street, London WC1E 6JJ, UK
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
October 20 1988
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1989 Plenum Publishing Corporation
1989
Biosci Rep (1989) 9 (1): 99–109.
Article history
Received:
October 20 1988
Citation
P. E. R. Tatham, B. D. Gomperts; ATP inhibits onset of exocytosis in permeabilised mast cells. Biosci Rep 1 February 1989; 9 (1): 99–109. doi: https://doi.org/10.1007/BF01117516
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |