A monospecific inhibitory antibody directed to phospholipase C (phosphoinositidase C) blocked the antiviral effect of human interferons alpha and beta when tested on human quiescent fibroblasts challenged with the vesicular stomatitis virus. This action was due to specific inhibition of polyphosphoinositide hydrolysis because (a) the F(ab′)2 fragment of the antibody molecule was also inhibitory; (b) excess antibodies directed to phospholipase A2 and to a phosphatidylcholine-preferring phospholipase C did not have any inhibitory effect, and (c) the combination of 12-O-tetradecanoylphorbol-acetate and calcium ionophore A23187 had an interferon-like antiviral effect which was not influenced by the inhibitory anti-phospholipase C antibodies. To avoid an interferon-like effect due to induction of interferon by second messengers, Vero cells, which lack interferon biosynthesis, were also used. Liposomes containing inositol 1,4,5-triphosphate and 1-oleoyl-2-acetyl-rac-glycerol protected Vero cells against the infection with the vesicular stomatitis virus. These results taken together show that phosphoinositide-derived second messengers are involved in triggering the antiviral effect of interferons alpha and beta.
Skip Nav Destination
Article navigation
Rapid Communication|
October 01 1989
Cell-membrane phospholipase C is involved in inducing the antiviral effect of interferon
L. M. Popescu;
L. M. Popescu
1Division of Cell Biology, Faculty of Medicine, Bucharest 35, P.O. Box 35-10, Romania
Search for other works by this author on:
C. Cernescu;
C. Cernescu
2Nicolau Institute of Virology, Bucharest, Romania
Search for other works by this author on:
I. I. Moraru;
I. I. Moraru
1Division of Cell Biology, Faculty of Medicine, Bucharest 35, P.O. Box 35-10, Romania
Search for other works by this author on:
St. N. Constantinescu;
St. N. Constantinescu
1Division of Cell Biology, Faculty of Medicine, Bucharest 35, P.O. Box 35-10, Romania
2Nicolau Institute of Virology, Bucharest, Romania
Search for other works by this author on:
F. Baltã;
F. Baltã
1Division of Cell Biology, Faculty of Medicine, Bucharest 35, P.O. Box 35-10, Romania
Search for other works by this author on:
M. Manciulea;
M. Manciulea
3Department of Immunology, Victor Babes Institute, Bucharest, Romania
Search for other works by this author on:
E. Brãiloiu;
E. Brãiloiu
4Department of Physiology, University of Craiova, Craiova, Romania
Search for other works by this author on:
L. Buzilã
L. Buzilã
5Department of Immunochemistry, Institute of Biological Sciences, Bucharest, Romania
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
December 19 1988
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1989 Plenum Publishing Corporation
1989
Biosci Rep (1989) 9 (5): 531–539.
Article history
Received:
December 19 1988
Citation
L. M. Popescu, C. Cernescu, I. I. Moraru, St. N. Constantinescu, F. Baltã, M. Manciulea, E. Brãiloiu, L. Buzilã; Cell-membrane phospholipase C is involved in inducing the antiviral effect of interferon. Biosci Rep 1 October 1989; 9 (5): 531–539. doi: https://doi.org/10.1007/BF01119795
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |