The influence of interferon alpha and gamma alone or in combination on the augmentation of human natural cytotoxicity was studied. Treatment of peripheral blood lymphocytes with IFN-α led to a rapid augmentation of NK activity, in contrast to IFN-γ where target cell killing was observed only following 18 hrs exposure of lymphocytes to IFN-γ. The results of the single cell assay paralleled those obtained using the Chromium release test, but neither interferon type caused an increase in the number of target binding lymphocytes. The combined effect of IFN-α and IFN-γ in stimulating human natural cytotoxicity demonstrated individual lymphocyte responses to be variable. Exposure of lymphocytes to IFN-α and IFN-γ for 18 hrs prior to assay for cytotoxicity usually decreased the level of cytotoxicity compared with control values, whereas other treatment regimes gave an additive and sometimes synergistic effect. Only treatment with IFN-α for 18 hrs and IFN-γ for one hr produced a synergistic response in the majority of individuals tested. We conclude from this study that individual responses to IFN-α and IFN-γ alone or in combination are variable and dependent upon timing of exposure of lymphocytes to individual interferon types, and possibly reflects the donor status at the time of sampling.
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October 01 1989
The influence of interferon alpha and gamma, singly or in combination on human natural cell mediated cytotoxicity
Lesley Rawlinson;
Lesley Rawlinson
1Department of Surgery, The University of Sheffield Medical School, Sheffield, UK
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Barbara J. Dalton;
Barbara J. Dalton
3Department of Immunology and Anti-infectives Therapy, Smith Kline and French Laboratories, Philadelphia, USA
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Kenneth Rogers;
Kenneth Rogers
1Department of Surgery, The University of Sheffield Medical School, Sheffield, UK
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Robert C. Rees
Robert C. Rees
2Department of Virology, The University of Sheffield Medical School, Sheffield, UK
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Publisher: Portland Press Ltd
Received:
January 10 1989
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1989 Plenum Publishing Corporation
1989
Biosci Rep (1989) 9 (5): 549–557.
Article history
Received:
January 10 1989
Citation
Lesley Rawlinson, Barbara J. Dalton, Kenneth Rogers, Robert C. Rees; The influence of interferon alpha and gamma, singly or in combination on human natural cell mediated cytotoxicity. Biosci Rep 1 October 1989; 9 (5): 549–557. doi: https://doi.org/10.1007/BF01119797
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