To explore the nature of differences in uptake by renal brush border vesicles from animals of different ages, vesicles were isolated from 7-day old and adult rats by a Mg-aggregation method. A number of criteria were compared in vesicles from the young and mature animals. The vesicles isolated from animals of both ages appear similar on electron microscopy, in response to osmotic changes, and in uptake kinetics for L-glucose. Despite these parameters which indicate no basic differences between the membranes of young and mature kidney, differences in proline and sodium handling are seen. When compared to the uptake pattern seen in vesicles from adult animals, the height of the sodium gradient-stimulated proline overshoot is diminished and sodium entry is faster in vesicles of the 7-day old rats. These are the same differences which were found in vesicles prepared by differential centrifugation from 7-day old animals. In addition, although sodium efflux was faster from vesicles of immature kidney and mirrored the faster sodium entry, proline efflux was slower. The data indicate a dissociation of proline and sodium fluxes in brush borders of the young rat kidney.
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December 01 1989
Characteristics of L-proline and sodium transport in renal brush border membranes isolated from 7-day-old and adult rats
Betty Y. L. Hsu;
Betty Y. L. Hsu
1Division of Biochemical Development & Molecular Diseases, Children's Hospital of Philadelphia, 34th Street & Civic Center Boulevard, Philadelphia, PA 19104, USA
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Pamela D. McNamara;
Pamela D. McNamara
1Division of Biochemical Development & Molecular Diseases, Children's Hospital of Philadelphia, 34th Street & Civic Center Boulevard, Philadelphia, PA 19104, USA
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Christina M. Cariola;
Christina M. Cariola
1Division of Biochemical Development & Molecular Diseases, Children's Hospital of Philadelphia, 34th Street & Civic Center Boulevard, Philadelphia, PA 19104, USA
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Elizabeth A. Fenstermacher;
Elizabeth A. Fenstermacher
1Division of Biochemical Development & Molecular Diseases, Children's Hospital of Philadelphia, 34th Street & Civic Center Boulevard, Philadelphia, PA 19104, USA
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Claire T. Rea;
Claire T. Rea
1Division of Biochemical Development & Molecular Diseases, Children's Hospital of Philadelphia, 34th Street & Civic Center Boulevard, Philadelphia, PA 19104, USA
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Robert A. Reynolds;
Robert A. Reynolds
1Division of Biochemical Development & Molecular Diseases, Children's Hospital of Philadelphia, 34th Street & Civic Center Boulevard, Philadelphia, PA 19104, USA
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Stanton Segal
Stanton Segal
1Division of Biochemical Development & Molecular Diseases, Children's Hospital of Philadelphia, 34th Street & Civic Center Boulevard, Philadelphia, PA 19104, USA
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Publisher: Portland Press Ltd
Received:
May 15 1989
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1989 Plenum Publishing Corporation
1989
Biosci Rep (1989) 9 (6): 709–719.
Article history
Received:
May 15 1989
Citation
Betty Y. L. Hsu, Pamela D. McNamara, Christina M. Cariola, Elizabeth A. Fenstermacher, Claire T. Rea, Robert A. Reynolds, Stanton Segal; Characteristics of L-proline and sodium transport in renal brush border membranes isolated from 7-day-old and adult rats. Biosci Rep 1 December 1989; 9 (6): 709–719. doi: https://doi.org/10.1007/BF01114809
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