Partitioning in charge-sensitive dextran-poly(ethylene glycol) aqueous phase systems reveals that fixation with even small concentrations of glutaraldehyde (e.g., 0.1% w/v) changes the surface properties of cells. While fixation with larger concentrations of glutaraldehyde (i.e., 1.85%) increases erythrocyte partition ratios, the effect of lower glutaraldehyde concentrations on the partition ratios appears to be species-specific. The differential effect of glutaraldehyde on rat reticulocytes and erythrocytes indicates that fixation is also cell-dependent. These data, together with the previous report that glutaraldehyde fixation does not change the characteristic relative partition ratios of rat mature erythrocytes of different cell ages, suggest that the nature and extent of glutaraldehyde alteration of cell surfaces must, in each case, be empirically evaluated.
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December 01 1989
Fixation with even small quantities of glutaraldehyde affects red blood cell surface properties in a cell- and species-dependent manner. Studies by cell partitioning
Harry Walter;
Harry Walter
1Laboratory of Chemical Biology, Veterans Administration Medical Center, Long Beach, CA 90822
2Department of Physiology and Biophysics, University of California, Irvine, CA 92717
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Eugene J. Krob
Eugene J. Krob
1Laboratory of Chemical Biology, Veterans Administration Medical Center, Long Beach, CA 90822
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Publisher: Portland Press Ltd
Received:
May 30 1989
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1989 Plenum Publishing Corporation
1989
Biosci Rep (1989) 9 (6): 727–735.
Article history
Received:
May 30 1989
Citation
Harry Walter, Eugene J. Krob; Fixation with even small quantities of glutaraldehyde affects red blood cell surface properties in a cell- and species-dependent manner. Studies by cell partitioning. Biosci Rep 1 December 1989; 9 (6): 727–735. doi: https://doi.org/10.1007/BF01114811
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