Apolipoprotein E deletion has no effect on copper-induced oxidative stress in the mice brain

The current study was designed to investigate effect of copper administration on oxidative damage to the brain in ApoE−/− mice and to explore the putative neuroprotective effects rendered by apolipoprotein E (ApoE). Male C57BL/6 ApoE−/− and wild-type mice were randomly assigned into four groups, ApoE−/− mice wild-type mice treated with either copper or saline. Copper sulphate pentahydrate or saline (200 µl) were administered intragastrically daily for 12 weeks. Expression of malondialdehyde, superoxide dismutase (SOD), hemeoxygenase 1 (HO-1), and NAD(P)H: quinone oxidoreductase 1 (NQO1) were determined by a combination of biochemical assays. The concentration of copper in the brain of C57BL/6 mice and ApoE−/− mice treated by copper significantly increased compared with mice treated by saline (P=0.0099 and P=0.0443). Compared with the C57BL/6 mice treated by copper, the level of the ApoE−/− mice treated by copper was higher (P=0.018). TBARS and SOD activities or the expressions of NQO1 and HO-1 in the brain were not significantly different amongst the four experimental groups of mice. The relative value of NQO1/β-actin expression in the brain of the ApoE−/− mice was similar in both saline and copper administration experimental groups. However, Western blot analysis showed that NQO1 expression was significantly higher in the ApoE−/− mice brain treated with saline compared with saline treated wild-type mice (P=0.0449). ApoE does not function in protecting the brain from oxidative damage resulting from copper build-up in Wilson’s disease, but may play a role in regulating copper accumulation in the brain.


Introduction
Hepatolenticular degeneration, also termed as Wilson's disease is an autosomal recessive genetic disorder and is characterized by ATP7B gene mutations, leading to defective copper metabolism [1]. Wilson's disease occurs approximately amongst 1 in 30000 live births [2]. The H1069Q mutation in European populations and the R778L mutation in Asian populations are the most common ATP7B mutation observed in patients with Wilson's disease [2][3][4][5].
Hepatic damage to various extents, neuropsychiatric symptoms, Kayser-Fleischer rings, and damage to the kidney and skeletal muscle are classical clinical hallmarks in Wilson's disease. Interestingly, liver damage is generally the major clinical manifestation at disease onset in children, whereas neurological symptoms tend to occur in those aged in their twenties or older [6]. There are though some reports of pediatric cases where primary symptoms were neurological, either isolated or along with mild hepatic injury. The diversity of symptoms and differences in age at disease onset suggest that the features of this disease may not be determined exclusively by the ATP7B gene mutations and that other genetic factors may be involved in the pathogenesis and perhaps progression of the disease.
Recently, some studies have shown that ages of onset as well as diversity of clinical presentation are correlated to the variant apolipoprotein E (ApoE) genotypes [7][8][9]. There are three alleles of the ApoE gene -ε2, ε3, and ε4 -encoding three different ApoE isoforms, each with biological functions different from the other [10]. Several lines of evidence have proved beyond doubt that the ApoE genotypes are associated with occurrence and clinical outcome of neurodegenerative diseases [10][11][12][13][14]. Whereas the ApoE 3 protein has neuroprotective function, the ApoE 4 protein increases vulnerability to CNS damage [15][16][17]. Because ApoE proteins can exert an antioxidant effect [18], and high copper build-up in Wilson's disease induce tissue damage via generation of free oxygen radicals [1], it is speculated that ApoE may render a neuroprotective effect in patients with Wilson's disease.
We have earlier determined the role of ApoE post copper administration induction of hepatic toxicity by modeling the ApoE −/− mice. We confirmed that copper accumulation can induce hepatic oxidative damage and that ApoE may protect the liver from this oxidative injury [18]. Given that copper accumulation also occurs in the brain, the objective of the current study was to explore whether ApoE exerts neuroprotective effect against oxidative damage induced by copper accumulation in the brain.

Animals
Animal experiments were performed after obtaining approval from the Institutional Animal Care and Use Committee of Hebei Medical University, Hebei, China, as described before [20]. Male C57BL/6 ApoE −/− and wild-type mice (15.1 + − 1.38 g; Animal Care Center of Beijing Medical University, China) were used in the present study. Animals were maintained as described before [20]. Twenty-four wild-type or ApoE −/− mice were randomly assigned into two groups -either treated with saline or copper. Copper sulphate pentahydrate (200 μl; 200 ppm; lot# BCBG7381V, Sigma, U.S.A.) or 200 μl saline was intragastrically administered daily for 12 weeks. After 12 weeks, mice (n=8 per group) were killed, and blood was obtained by orbital bleeding. The brains were removed, snap-frozen, and stored in liquid nitrogen until further use. The remaining four mice from each group were processed for immunohistochemistry as described below.

Preparation of tissue samples
Brain tissue homogenates were prepared using ice-cold PBS containing protease inhibitor cocktail (Sigma-Aldrich, U.S.A.) by homogenizer. Homogenates were centrifuged at 8000 g for 10 min. TBARS and superoxide dismutase (SOD) activities were determined in the supernatants as described in the next section.

Determination concentration of copper in brain
The concentration of copper was determined according to the method as described in previous study [20]. Two hundred milligrams of brain samples were digested using 5 ml of concentrated nitric acid and 1 ml of 30% H 2 O 2 in a microwave digestion system (CEM, U.S.A.). And all samples were heated to 140 • C until the volume declined to 2 ml after they cooled down. Each sample was washed for three times using 1% nitric acid. The atomic absorption spectrometer was used to determine the concentration of copper.

Determination of TBARS and SOD activities in the brain
TBARS activities were determined using the thiobarbituric acid (TBA) method (Nanjing Jiancheng Bioengineering Institute, China) as described before [20]. The concentration of TBARS was counted as nM of TBARS per mg of protein. SOD activities were determined by the xanthine oxidase method as described previously [20], and the results were presented as units per milligram of protein.

Immunohistochemistry
After copper or saline administration for 12 weeks, four mice were randomly selected from each group for immunohistochemical experiments using SABC three-step kit (Boster, Wuhan, China). Intraperitoneal injection of 10% chloral hydrate (300 mg/kg) was used for anesthesia. Post-anesthesia mice were transcardially perfused with 200 ml of normal saline followed by 4% paraformaldehyde in PBS. The brains were removed and fixed for 24 h in 4% paraformaldehyde, and embedded in paraffin. Tissue sections (5 μm) were obtained using a microtome. Treatment with 3% H 2 O 2 at room temperature for 15 min was done to block the endogenous peroxidase activity. Sections were then processed as described previously [20] and incubated with primary antibodies against hemeoxygenase 1 (HO-1; 1:100 dilution, Epitomics, U.S.A.) or NAD(P)H: quinone oxidoreductase 1 (NQO1; 1:100 dilution, Epitomics, U.S.A.) overnight at 4 • C. Sections were developed using DAB staining and were then counterstained with Hematoxylin. The sections not containing primary antibodies were used as negative controls. A light microscope was used to image the immunostained sections.

Western blot
Immunoblot analysis of proteins expressed in brain was assayed in lysates made from brain tissues as described before [20].

Quantitative real-time PCR
Total RNA was isolated with TRIzol reagent (Thermo Fisher Scientific, Shanghai, China) and treated with DNase to get rid of any contaminating genomic DNA. cDNA and quantitative real-time PCR (qRT-PCR) analysis were performed as described before [20]. Primers used were NQO1 forward primer 5-TATGCTGCCATGTACGACAACGG-3, reverse primer 3-AAGACCTGGAAGCCACAGAAACG-5. Data analysis was performed using the Sequence Detection Systems interface (Thermo Fisher Scientific).

Statistical analysis
Data were expressed as mean + − S.D. Two-way ANOVA was used for comparing continuous data between male C57BL/6 ApoE −/− group and wild-type mice group that expression of malondialdehyde, SOD, HO-1, and NQO1 data, which were determined by a combination of biochemical assays. If the interaction was significant, least square mean test was used for pairwise comparison only if interaction was statistically significant. All statistical analysis was performed with SPSS 21.0 software and P<0.05 was considered as statistically significant.

Results
The concentration of copper in the brain of ApoE −/− mice To verify the concentration of copper in the brain of the four groups, we determined the concentration of copper used the atomic absorption spectrometer. The results showed that ApoE −/− mice treated with saline exhibited significantly higher copper concentrations in the brain compared with the wild-type mice treated with saline (4.3 + − 0.52 compared with 2.5 + − 0.43; P=0.0099). The copper concentration of C57BL/6 mice after treatment of copper was significantly higher compared with the C57BL/6 mice treated by saline in the brain tissue

The expression of NQO1 mRNA in the brain
To determine the expression of NQO1, we also used the qRT-PCR to detect the level of NQO1 in ApoE −/− mice. Even though the relative ratio of NQO1 and ACTB (encoding β-actin) in the ApoE −/− mice brain treated with copper was slightly more than the ApoE −/− mice treated with saline, the difference did not attain statistical significance (1.4 + − 1 compared with 0.8 + − 0.12; P=0.3605; Figure 4).

Discussion
Clinical outcomes in vascular and neurodegenerative disorders seem to be regulated by the ApoE genotypes [14,19,20]. In Alzheimer's disease, the ApoE 3 protein functions as a neuroprotector; in comparison, the ApoE 4 protein promotes CNS injury [15][16][17]. Several studies have shown that APOE ε4-positive genotype is associated with  earlier symptomatic visibility of Wilson's disease, particularly amongst patients harboring the ATP7B p.H1069Q homozygous patients in women [7][8][9]. However, whether ApoE facilitates neuroprotection in Wilson's disease was not known.
In an earlier study [20], we showed the role of ApoE in copper buildup-induced oxidative injury in the liver by administering copper for 12 weeks at high dosage of 200 mg/kg/day to ensure effectiveness of copper toxicity. The animals treated with copper exhibited a significantly higher copper concentration in the liver than the animals treated with saline, suggesting that excessive copper accumulates in the liver. Furthermore, copper treatment also resulted in excessive copper accumulation in other organs including the brain and kidney. Thus, this animal model exhibited copper build-up in the brain, kidney, and liver with wide-ranging hepatic damage, which mimics Wilson's disease presentation features. We found that copper induced an elevation in TBARS activities and an inhibition in SOD activities in the serum and liver respective of ApoE expression, accompanied by an elevation in the expression of NQO1 and HO-1, indicating that elevation of copper concentration can induce oxidative damage in the liver.
Copper functions as the cofactor of various enzymes participating in a diverse array of cellular processes. Therefore, it is likely that pleiotropic mechanisms regulate copper toxicity. High levels of copper were associated with neural degeneration, reducing the number of nerve cells [21], and miniature quantities of copper-induced neurotoxicity in cholesterol-fed mice via oxidative stress-induced apoptosis [22].
In this study, the mice were also exposed to copper administration for 12 weeks with the high dosage of 200 mg/kg/day to ensure effectiveness of copper toxicity. In toxicologic studies, copper is usually administered in doses of 50 mg/kg/day as low toxicity, 100 mg/kg/day as moderate toxicity, and 200 mg/kg/day as high toxicity [23]. On the other hand, it has been reported that, hepatic injury might occur after a period of 12 weeks feeding with standard chow diet [24,25]. In the 12 weeks, the copper accumulated in the brain and the hepatic injury was not very serious. In light of the above, 12 weeks of copper in high dosage was chosen. We found that the ApoE −/− mice treated with saline exhibited significantly higher copper concentrations in the brain compared with the wild-type mice treated with saline, and the ApoE −/− mice treated with copper sulphate pentahydrate also exhibited significantly higher copper concentrations in the brain compared with the wild-type mice treated with copper sulphate pentahydrate, suggesting that ApoE may participate in regulating copper accumulation in the brain. However, it remains unknown how ApoE knockout results in copper accumulation in the brain. The blood-brain barrier is disrupted in ApoE −/− mice [26] resulting in the possibility that increase in the permeability of the blood-brain barrier to copper may lead to an increase in brain copper concentrations in ApoE −/− mice. However, though brain copper concentrations were significantly higher in the wild-type mice treated with copper and the ApoE −/− mice treated with saline or copper compared with the wild-type mice treated with saline, no significant differences in TBARS and SOD activities or the expression of NQO1 and HO-1 were found amongst the saline or copper treated wild-type mice and ApoE −/− mice.
Even though copper deposited in the liver is thought to initiate Wilson's disease, the disease presentation does not match with degree of copper accumulation in the liver, indicating that a multitude of factors may contribute significantly to Wilson's disease pathology [27]. These factors include the exposure time to copper, the intracellular copper distribution, the presenting form of copper in the liver, and the involvement of additional protein regulators. Due to the expression of ATP7B in the CNS, the CNS abnormalities may be accompanied by liver damage, and the delay of neurological symptoms may be arising from partial compensation of ATP7B malfunction by another copper-transporting ATPase, ATP7A, which is expressed in the brain, but not in the liver cells.
To further investigate the impact of ApoE on the brain, we detected both the expression of NQO1 mRNA, and the relative value of NQO1/β-actin in the brain. The results by the two tests were in accordance, showing no differences between the ApoE −/− mice with either, suggesting that ApoE may not produce neuroprotective roles in copper-induced oxidative damage further.
In summary, our results showed that knockout of ApoE potentiated copper buildup-induced oxidative damage in the brain. However, although the brain copper concentrations increased in the ApoE −/− mice, no significant differences in the TBARS and SOD activities or the expression of NQO1 and HO-1 were found in the copper treated ApoE −/− mice. Our findings suggest that ApoE may not protect the brain from copper-induced oxidative damage but might play a yet undefined role in regulating copper accumulation in the brain.

Funding
This work was supported by the Science and Technology Program Project of Hebei Province [grant number 162777197].