Zn2+ can induce a conformational change of Band 3 with concomitant inhibition of its anion transport activity of human erythrocyte membrane vesicles only from the cytoplasmic side. The Zn2+ inhibition exhibits a dose-dependent manner with an apparent half maximal concentration of 50 μM ZnCl2 and can be reversed by 0.5 mM EDTA, but not by 1 mM dithiothreitol. The Zn2+ effect is specific and no similar inhibitory action could be observed by other divalent cations (Cu2+, Mn2+, Mg2+ or Sr2+).

Abbreviations: DPA, dipicolinic acid; EITC, eosin 5-isothiocyanate; DIDS, 4,4′-diisothiocyanostilbene-2,2′-disulfonate; TES, N-Tris-(hydroxymethyl)methyl-2-aminoethane sulfonic acid; EDTA, ethylendiamine tetraacetic acid; DTT, dithiothreitol; NEM, N-ethylmaleimide; EITC-Band 3, Band 3 labeled with EITC

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