In the present work we used various cell lines in order to study the possible effect of coxsackievirus B3 (CVB3) entry on the adenylyl cyclase transmembrane signalling system. A significant decrease (by about 10–20%) was found in forskolin-augmented as well as in AlF4−- and GTPγS-sensitive adenylyl cyclase activity in plasma membranes isolated from HeLa, HEp-2, Vero and green monkey kidney cells shortly (up to 60 min) preincubated with CVB3 (5 PFU/cell). Moreover, the ability of G-proteins derived from plasma membranes of infected cells to reconstitute AC activity in the cyc− mutant of S49 cells was also reduced. Content of G-protein subunits, however, remained unchanged after CVB3 attachment. Functional alterations in the G-protein-mediated adenylyl cyclase signalling system were accompanied by a marked decrease (by about 20–40%) of intracellular cAMP levels in virus-affected cells. These findings demonstrate clearly that CVB3 may affect functioning of the G-protein regulated adenylyl cyclase transmembrane signalling system in virus-sensitive cells as early as during the first period of its contact with the cellular plasma membrane.
Coxsackievirus B3 entry into the host cell interferes with G-protein-mediated transmembrane signalling
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Jiri Novotny, Petr Kvapil, Jeronimo Cello, Lennart A. Ransnäs; Coxsackievirus B3 entry into the host cell interferes with G-protein-mediated transmembrane signalling. Biosci Rep 1 August 1994; 14 (4): 205–214. doi: https://doi.org/10.1007/BF01200249
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