In the present work combined glycan-, lectin-, and immunoblotting of isolated brain and kidney membranes shows that the α and β subunits of Na,K-ATPase are the most abundant glycoproteins. Further,Datura stramonium and Galanthus nivalis agglutinins recognize the Na,K-ATPase subunits in a mutually exclusive manner in membranes from human, rabbit and rat brain or human, rabbit, rat, pig and dog kidney indicating the presence of species-independent organ-typical glycoforms. The glycosylation status is not related to the ouabain-sensitivity. Taken together, the data reveals organ-specific glycoforms of Na,K-ATPase which might have roles for organ identification and recognition.
Abbreviations NKA, Na,K-ATPase (EC 184.108.40.206); PAGE, polyacrylamide gel electrophoresis in dodecylsulfate; Con-A, Concanavalin A; DSA, Datura stramonium agglutinin; GNA, Galanthus nivalis agglutinin; MAA, Maackia amurensis agglutinin; PNA, Peanut agglutinin; SNA, Sambucus nigra agglutinin; WGA, Wheat germ agglutinin;
Abbreviations used in figures K, kidney; B, brain; Cr, Crude; De, Detergent-treated; Fe, fetuin; Ct, creatinase; I-blot, immuno-blot; L-blot, lectin-blot;