The relationship between Ca2+ transport and energy transduction of myocardial mitochondria in the presence of reactive oxygen species was investigated. Following treatment with oxygen free radicals [superoxide(O2•) or hydroxyl radical (•)OH], lipid free radicals in myocardial mitochondrial membrane could be detected by using the method of EPR spin trap. Simultaneously there were obvious alterations in the free Ca2+ ([Ca2+]m) in the mitochondrial matrix; the physical state of membrane lipid; the efficiency of oxidative phosphorylation (ADP/O); the value of the respiratory control ratio (RCR); and the membrane potential of the inner membrane of myocardial mitochondria. If the concentrations of reactive oxygen species were reduced by about 30%, the alterations in the physical state of the membrane lipid and energy transduction of myocardial mitochondria were not observed, but the changes in Ca2+ homeostasis remained. We conclude that Ca2+ transport by myocardial mitochondria is more sensitive to agents such as (O2•) or •OH, etc. than are oxidation phosphorylation and the respiratory chain.
Research Article| December 01 1997
Sensitivity of Ca2+ Transport of Mitochondria to Reactive Oxygen Species
Biosci Rep (1997) 17 (6): 557–567.
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
Zhi-wei Yang, Fu-yu Yang; Sensitivity of Ca2+ Transport of Mitochondria to Reactive Oxygen Species. Biosci Rep 1 December 1997; 17 (6): 557–567. doi: https://doi.org/10.1023/A:1027316424985
Download citation file: