In this paper we report the successful expression of the winged bean basic agglutinin (WBA I) in insect cells infected with a recombinant baculovirus carrying the WBA I gene and its characterization in terms of its carbohydrate binding properties. The expressed protein appears to have a lower molecular weight than the native counterpart which is consistent with the lack of glycosylation of the former. Moreover, the expressed protein maintains its dimeric nature. Hence, a role for glycosylation in modulation of dimerization of WBA I is ruled out unlike Erythrina corallodendron (EcorL). Despite this the protein is active, with its sugar specificity unaltered.
Expression of Winged Bean Basic Agglutinin in Spodoptera frugiperda Insect Cell Expression System
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
V. R. Srinivas, Kiran Bachhawat-Sikder, Saman Habib, Seyed E. Hasnain, Avadhesha Surolia; Expression of Winged Bean Basic Agglutinin in Spodoptera frugiperda Insect Cell Expression System. Biosci Rep 1 June 2001; 21 (3): 361–367. doi: https://doi.org/10.1023/A:1013294417847
Download citation file: