Cell-swelling, induced by a hyposmotic challenge, stimulated the efflux of L-carnitine from a human mammary cancer cell line, MDA-MB-231. The response was dependent upon the extent of the osmotic shock. Hyposmotically-activated L-carnitine efflux was inhibited by the anion transport blocker diiodosalicylate. The efflux of taurine from MDA-MB-231 cells was also stimulated by a hyposmotic shock via a pathway sensitive to diiodosalicylate. L-carnitine efflux from MDA-MB-231 cells was stimulated by isosmotic swelling in a manner which was inhibited by diiodosalicylate. The results suggest that L-carnitine may exit cells via a volume-sensitive pathway: it is possible that L-carnitine efflux may utilize the same pathway as amino acids. The efflux of L-carnitine via this route could have a major effect on the intracellular concentration of L-carnitine and could facilitate transepithelial L-carnitine transport.
Research Article| December 01 2001
Hyposmotically-Activated Efflux of L-Carnitine from a Human Mammary Cancer Cell Line
Biosci Rep (2001) 21 (6): 779–787.
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D.B. Shennan; Hyposmotically-Activated Efflux of L-Carnitine from a Human Mammary Cancer Cell Line. Biosci Rep 1 December 2001; 21 (6): 779–787. doi: https://doi.org/10.1023/A:1015584724234
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