Using surface-enhanced infrared absorption (SEIRA) spectroscopy of dry films of colloidal gold (CG) bioconjugates with protein A, it is shown that certain characteristic bands of the protein (e.g., amide I, amide II and some other vibration modes) are essentially affected by the metal surface. Thus, the method may be used for controlling the quality of such bioconjugates. Moreover, it is demonstrated that the biospecific reaction of protein A attached to CG particles with human immunoglobulin G (IgG) results in further essential changes in SEIRA spectra, providing a means for an easy and rapid IR spectroscopic detection of biospecific immunochemical interactions (i.e., spectroimmunochemistry). The results obtained can form a basis for developing test systems for detecting various biospecific interactions.
Spectroimmunochemistry Using Colloidal Gold Bioconjugates
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Alexander A. Kamnev, Lev A. Dykman, Petros A. Tarantilis, Moschos G. Polissiou; Spectroimmunochemistry Using Colloidal Gold Bioconjugates. Biosci Rep 1 December 2002; 22 (5-6): 541–547. doi: https://doi.org/10.1023/A:1022077920527
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