Phosphatidylinositol (Ptd Ins) breakdown in response to thyrotropin-releasing hormone (TRH) was measured after preincubation of both normal rat anterior pituitary cells and GH3 turnout cells with [3H]inositol by the determination of [3H]inositol phosphate accumulation in the presence of lithium (which inhibits myo-inositol phosphatase). The method employed, which was originally developed for use with tissue slices, was adapted for isolated cells in monolayer culture. In GH3 cells, TRH stimulated the breakdown of phosphoinositide in a manner similar to that reported previously using alternative methods. Furthermore, in normal male anterior pituitary cells the dose-response profile for TRH stimulation of inositol phosphate accumulation was found to correlate well with the dose-response profile for TRH stimulation of prolactin secretion. As this response was maintained in the absence of added calcium, the breakdown of phosphoinositide would appear to be implicated as an event preceding calcium mobilization.
Skip Nav Destination
Article navigation
Research Article|
December 01 1983
Thyrotropin-releasing hormone stimulates inositol phosphate production in normal anterior pituitary cells and GH3 tumour cells in the presence of lithium
John G. Baird;
John G. Baird
1Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, U.K.
Search for other works by this author on:
Pauline R. M. Dobson;
Pauline R. M. Dobson
1Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, U.K.
Search for other works by this author on:
Richard J. H. Wojcikiewicz;
Richard J. H. Wojcikiewicz
1Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, U.K.
Search for other works by this author on:
Barry L. Brown
Barry L. Brown
1Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School, Beech Hill Road, Sheffield S10 2RX, U.K.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
October 03 1983
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1983 The Biochemical Society
1983
Biosci Rep (1983) 3 (12): 1091–1099.
Article history
Received:
October 03 1983
Citation
John G. Baird, Pauline R. M. Dobson, Richard J. H. Wojcikiewicz, Barry L. Brown; Thyrotropin-releasing hormone stimulates inositol phosphate production in normal anterior pituitary cells and GH3 tumour cells in the presence of lithium. Biosci Rep 1 December 1983; 3 (12): 1091–1099. doi: https://doi.org/10.1007/BF01120201
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.