125I-insulin was shown to be internalized in vivo to a discrete population of low-density membranes (ligandosomes), distinct from the Golgi, endoplasmic reticulum, plasma membrane, and lysosomes. However, analytical subcellular fractionation shows that glutathione-insulin transhydrogenase is localized to the endoplasmic reticulum. Measurement of the specific enzyme activity of glutathione-insulin transhydrogenase showed no differences between normal, diabetic, and hyperinsulinaemic rats. These results suggest that glutathione-insulin transhydrogenase is not directly involved in the subceltular processing of receptor-bound internalized insulin.
Studies on the subcellular localization and role of glutathione-insulin transhydrogenase in rat liver
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Balvinder K. Chowdhary, Geoffrey D. Smith, Robert Mahler, Timothy J. Peters; Studies on the subcellular localization and role of glutathione-insulin transhydrogenase in rat liver. Biosci Rep 1 April 1983; 3 (4): 323–329. doi: https://doi.org/10.1007/BF01122896
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