The translation strategy of carnation mottle virus (CarMV) in vitro has been generally assumed to involve internal initiation events on full-length, genomic RNA (4.3 kb). We suggest that this is, at least in part, incorrect. Encapsidated RNA, fractionated on denaturing sucrose gradients, or total RNA from CarMV-infected leaves, fractionated under non-denaturing conditions, was translated in an mRNA-dependent rabbit reticulocyte cell-free system. Evidence for subgenomic RNAs which encode a polypeptide of Mr 38 000 was found. This product was shown to be related to authentic CarMV coat protein by partial proteolysis with α-chymotrypsin and SDS/polyacrylamide-gel electrophoresis.
An encapsidated, subgenomic messenger RNA encodes the coat protein of carnation mottle virus
- Views Icon Views
- PDF LinkPDF
- Share Icon Share
- Cite Icon Cite
Sally-Ann Harbison, T. Michael A. Wilson, Jeffrey W. Davies; An encapsidated, subgenomic messenger RNA encodes the coat protein of carnation mottle virus. Biosci Rep 1 November 1984; 4 (11): 949–956. doi: https://doi.org/10.1007/BF01116893
Download citation file: