The erythroleukemia cell line K562 was previously shown to have specific binding sites for insulin but not for insulin-like growth factor I (IGF-I). In this study the presence of specific receptors for insulin-like growth factor II (IGFqI) is established. Scatchard analysis of the competition curve for IGF-II disclosed a non-cooperative binding kinetic with a calculated affinity constant of 2.4 × 108 M−1 and a receptor number of 4.8 × 104 sites/cell. IGF-I displayed 10% crossreactivity over the IGF-II receptor but insulin did not crossreact at all. Instead insulin, present in high concentrations, enhanced the binding of IGF-II. The presence of IGF II but not IGF-I receptors makes the K562 cell line suitable for studying properties of the type-2 receptor.
The binding of insulin-like growth factor II to the erythroleukemia cell line K562
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Michael Tally, Tang Xin-Zhi, Gösta Enberg, Kerstin Hall; The binding of insulin-like growth factor II to the erythroleukemia cell line K562. Biosci Rep 1 December 1984; 4 (12): 1071–1077. doi: https://doi.org/10.1007/BF01116701
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