A polyclonal antibody against rabbit cathepsin L was raised in goats and shown to be specific for both active and inactive enzyme. Using this antibody we have examined the distribution of cathepsin L in primary rabbit skin fibroblasts by immunohistochemistry and found that all the enzyme is located within lysosomal granules. At confluence many cathepsin-L-containing lysosomes were seen in each celt. A new but nonspecific histochemical substrate for cathepsin L was tested and a similar distribution was obtained. Our results indicate that the immunohistochemical technique can be reliably employed for the specific location of cathepsin L in cells and tissues.
Production of a monospecific antiserum to cathepsin L: The histochemical location of enzyme in rabbit fibroblasts
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David J. Etherington, Robert W. Mason, Mark A. J. Taylor, R. John Wardale; Production of a monospecific antiserum to cathepsin L: The histochemical location of enzyme in rabbit fibroblasts. Biosci Rep 1 February 1984; 4 (2): 121–127. doi: https://doi.org/10.1007/BF01120308
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