The present communication reports the occurrence of high-mannose oligosaccharides on Tamm-Horsfall glycoprotein prepared from human pooled urine. The Pronase digest of the glycoprotein was fractionated by gel filtration and a high-mannose glycopeptide species was separated from complex-type glycopeptides. When high-mannose glycopeptides were digested with endo-β-N-acetylglucosaminidase H, followed by reduction with [3H]KBH4, three oligosaccharides were resolved by thin-layer chromatography. On the basis of chromatographic mobility and exoglycosidase digestions the composition Man7-, Man6-, and Man5-GlcNAc was assigned to the three oligosaccharides. Man6GlcNAc is by far the major component.
Research Article| March 01 1984
High-mannose oligosaccharides from human Tamm-Horsfall glycoprotein
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Franca Serafini-Cessi, Fabio Dall'olio, Nadia Malagolini; High-mannose oligosaccharides from human Tamm-Horsfall glycoprotein. Biosci Rep 1 March 1984; 4 (3): 269–274. doi: https://doi.org/10.1007/BF01119663
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